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Tuesday, August 4, 2020 | History

2 edition of Adenosine metabolism in baby hamster cells. found in the catalog.

Adenosine metabolism in baby hamster cells.

Sherrill Maureen Archer

Adenosine metabolism in baby hamster cells.

by Sherrill Maureen Archer

  • 279 Want to read
  • 28 Currently reading

Published .
Written in English


The Physical Object
Pagination120 leaves
Number of Pages120
ID Numbers
Open LibraryOL16344180M

  HEK is the most extensively used human cell line for the production of viral vectors and is gaining increasing attention for the production of recombinant proteins by transient transfection. To further improve the metabolic characterization of this cell line, we have performed cultures using 13C-labeled substrates and measured the resulting mass isotopomer distributions in lactate by LC/MS. The action of endogeneous adenosine on isolated hamster brown adipocytes was examined. Adenosine production from brown adipocytes was measured after labeling of the intracellular nucleotide pool with [3H]adenine. Accumulation of [3H]adenosine in the incubation medium was maximum after 5 min of incubation and was still present after 20 min.

  1 Of 85 patients who received drug, 3 were not included in the efficacy analysis because they had. A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text.

One week after growth in neurogenic conditions, Adk −/ − NPs released ± ng adenosine/10 5 cells in 2 h, while the amount of adenosine released from wt cells, ± ng adenosine/10 5 cells in 2 h, was minimal. for adenosine transport or if both vascular cells function as a single compartment in the metabolism of adenosine. A possible mechanism proposed to explain the transfer of the adenosine vasodilator signal from vessel lumen across the endothelium to smooth muscle is that these two cellular compartments are metabolically coupled via gap junctions.


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Adenosine metabolism in baby hamster cells by Sherrill Maureen Archer Download PDF EPUB FB2

Adenosine is known to modulate cell growth in a variety of mammalian cells either via the activation of receptors or through metabolism. We investigated the effect of adenosine on Baby Hamster Kidney (BHK) cell growth and attempted to determine its mechanism of by: 2.

Biofactors. ;11(4) Adenosine modulates cell growth in baby hamster kidney (BHK) cells. Mittal RA(1), Tan CH, Khoo HE. Author information: (1)Department of Biochemistry, Faculty of Medicine, National University of Singapore, Singapore.

Adenosine is known to modulate cell growth in a variety of mammalian cells either via the activation of receptors or through by: 2. Adenosine diphosphate ribose transferase from baby-hamster kidney cells (BHK/C13).

(adenosine dephosphate ribose) metabolism and regulation of myocardial cell growth by oxygen. Biochem J. Feb 15; (2) STOKER M. Polyoma transformation of hamster cell clones--an investigation of genetic factors affecting cell by: 9. Juranka P, Chan VL () Analysis of adenosine kinase mutants of baby hamster kidney cells using affinity-purified antibody.

J Biol Chem – PubMed Google Scholar Kloor D, Hermes M, Fink K, Schmid H, Klingel K, Mack A, Grenz A, Osswald H () Expression and localization of S-adenosylhomocysteine-hydrolase in the rat kidney Cited by:   In recent years, several human cell populations and cell-derived structures have been shown to be involved in adenosine metabolism, including Treg, B-cells, T-helper-cells, cytotoxic T-cells, MSC, non-cellular exosomes and others (Saze et al.,Schuler et al.,Schuler et al., ).

Possibly, this may be a source for a future Cited by: Adenosine and its receptors are critical regulators of osteoclast differentiation. Adenosine A2B receptor is critical for osteoblast differentiation but the other three adenosine receptors also play a major role in bone formation.

Adenosine A2A receptor may be an important regulator of cartilage loss. Abstract. A class of arabinosyladenine-resistant baby hamster kidney (BHK) cell mutants, isolated in our laboratory, shows cross-resistance to deoxyadenosine, alteration of adenosine kinase, elevation of spontaneous mutation rate, and extreme sensitivity to adenosine.

An adenosine-sensitive (Ado s) mutant of baby hamster kidney (BHK) cells, ara-S10d, when treated with a toxic concentration of adenosine (Ado), displayed a substantial elevation of S-adenosylhomocysteine (SAH), S-adenosylmethionine (SAM), and methylthioadenosine (MTA).Wild-type BHK cells treated with the same concentration of Ado (not toxic to these parental cells) produced an.

Adenosine is an endogenous metabolite that is released from all tissues and cells including liver, pancreas, muscle and fat, particularly under stress, intense exercise, or during cell damage. The role of adenosine in glucose homeostasis has been attributed to its ability to regulate, through its membrane receptors, processes such as insulin.

Adenosine deamination also occurs in the plasma, but at a lower rate than that which occurs within cells. Dipyridamole is a vasodilator drug that blocks adenosine uptake by cells, thereby reducing the metabolism of adenosine.

Therefore, one important mechanism for dipyridamole-induced vasodilation is its potentiation of extracellular adenosine. Analysis of the response of baby hamster kidney cells to adenosine in the presence of the adenosine deaminase inhibitor erythro‐9‐(2‐hydroxy‐3‐nonyl) adenine has revealed two distinct mechanisms of toxicity.

The first is apparent at low concentrations of adenosine (adenosine. P1 or adenosine receptors. Adenosine is generated both intracellularly and extracellularly from the hydrolysis of adenine nucleotides (Figure 1), and acts locally to exert its extracellular physiologic and pharmacologic effects via activation of specific cell surface G protein coupled receptors (A 1, A 2A, A 2B and A 3), proteins with unique pharmacological profile, tissue distribution and.

9-β-D-Arabinosyladenine (araA)-resistant mutants of baby hamster kidney (BHK) cells can be classified into 3 order to gain a better understanding of the mechanism(s) of resistance and the biochemical basis of cytotoxicity of various purine nucleosides, cell hybrids of the mutant and wild-type cells were made and analyzed.

2 Adenosine Metabolism, Adenosine Ki nase, and Evolution involved in A TP-binding, on the other hand, include Asn, Asn, Glu, Thr, Gly, V al, and Ile, which provide fewer. Adenosine: A Key Link Between Metabolism and Central Nervous System Activity focusses on diverse aspects of adenosine, an evolutionarily conserved homeostatic bioenergetic regulator in the central nervous system.

Because of its interrelationship with ATP (adenosine triphosphate), adenosine is integral to cell metabolism. The antilipolytic action of adenosine is not only of pharmacological interest, but may play a physiological role.

It was shown early (Fain and Wieser,Hjemdahl and Fredholm, ), and has been confirmed repeatedly, that removal of adenosine from the medium by adenosine deaminase or antagonism of receptors by low doses of methylxanthines causes increased lipolysis (e.g., Fig.

Multiple mechanisms of adenosine toxicity in an adenosine sensitive mutant of baby hamster kidney (BHK) cells. Chan VL, Ho HJ. A class of arabinosyladenine-resistant baby hamster kidney (BHK) cell mutants, isolated in our laboratory, shows cross-resistance to deoxyadenosine, alteration of adenosine kinase, elevation of spontaneous mutation rate.

Biochemistry 8:Mudd SH, Activation of methionine for transmethylation. J Biol ChemKamely D, Littlefield JW and Erbe RW, Regulation of 5-methyl-tetrahydrofolate:homocysteine methyltransferase activity by methionine, vitamin B12, and folate in cultured baby hamster kidney cells.

Dieterle, C. Ody, A. Ehrensberger, H. Stalder, and A.F. Junod, Metabolism and uptake of adenosine triphosphate and adenosine by porcine aortic and pulmonary endothelial cells and fibroblasts in culture, Circ.

Res. PubMed CrossRef Google Scholar. Adenosine kinase (ADK) is the first enzyme in the adenosine remediation pathway that catalyzes adenosine phosphorylation into adenosine monophosphate, thus regulating adenosine homeostasis in cells. To obtain new insights into ADK from Bombyx mori (BmADK), we obtained recombinant BmADK, and analyzed its activity, structure, and function.

Gel-filtration showed BmADK was a monomer with. Adenosine is likely to act via specific receptors, located on the outer surface of the cell membrane of smooth muscle, cardiac muscle, and nodal cells [10, 11]. The adenosine receptors are linked to the adenylate cyclase system [5–7] and receptor activation can inhibit transmembrane Ca 2+ influx [12, 13].

To assess cross-species compatibility of acetaldehyde-inducible transgene expression, we seeded baby hamster kidney (BHK), CHO-K1 and human HeLa cells. Purinergic co-transmission might be involved in sympathetic control of BAT and previous studies reported inhibitory effects of the purinergic transmitter adenosine in BAT from hamster .